- PRODUCT DESCRIPTION
+ In the World Health Organization (WHO) classification of tumors of hematopoietic and lymphoid tissues, Philadelphia-negative myeloproliferative neoplasms (MPNs)
+ Include polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF).
+ In recent studies it was found that, three-quarters of patients carry the unique JAK2 (V617F) mutation, which is present in about 95% of subjects with PV and in about 60% of those with ET or PMF.
+ Somatic mutations of JAK2 exon 12 are found in the remaining 5% of patients with PV, whereas mutations of MPL exon 10 are present in about 5% of those with ET or PMF.
+ Most patients with ET or PMF with nonmutated JAK2 and MPL carry a somatic mutation of CALR, the gene encoding Calreticulin1.
+ Kit screens JAK-2 gene Exon 12 mutations;
+ between amino acids 530 and 547, JAK-2 Exon 14 gene V617F mutation, MPL gene Exon 10; S505N, W515A, W515K, W515RL, W515R mutations, CALR gene Exon 9;
+ Type 1, Type 2 and other mutations and CSF3R gene; T615A and T618I mutations.
+ Please check the mutation lists at the last page for detailed information about the mutations that can be screened with the kit.
+ The kit offers sensitivity to detect under 1% mutant allele in background of 99% wild type allele in the related mutations.
- PRINCIPLE OF THE SYSTEM
+ During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencer dye only when the probe hybridizes perfectly to the target DNA.
+ This cleavage results in the fluorescent signal which is monitored by Real-Time PCR detection system.
+ An increase in the fluorescent signal (CT) is proportional with the amount of mutant PCR products.
+ System gives amplification plots only if there is any mutation in the sample DNA except internal control.
+ Wild type sample amplifies only with internal control.
+ In order to analyze some regions, wild type sequences are blocked by specifically designed oligonucleotides.
+ It gives perfect opportunity to screen mutations, since there is not any prevention in mutant types.
